Journal: Frontiers in immunology
Article Title: Investigating mammary glands of lactating goats for the presence of tertiary lymphoid organs.
doi: 10.3389/fimmu.2022.941333
Figure Lengend Snippet: FIGURE 1 Representative images of hematoxylin and eosin staining (HE) and immunohistochemistry against CD20 (B cells), CD3 (T cells), MECA79 (high endothelial venules; HEVs), CD40 (follicular dendritic cells), and BCL6 (germinal center) in a small-sized aggregation of lymphocytes in goat mammary gland tissues. Scale bar, 100 mm or 25 mm (clippings).
Article Snippet: The sections were then incubated overnight at 4°C with either rabbit polyclonal antibodies against goat-IgA (#A50-106A, Bethyl Laboratories), claudin-3 (#34-1700, Thermo Fisher Scientific), claudin-4 (#PA5-32354, Thermo Fisher Scientific), or mouse monoclonal antibodies against BCL6 (#sc7388, Santa Cruz Biotechnology), CD3 (#NBP2-53386, Novus Biologicals), CD20 (#NBP2-45454, Novus Biologicals), CD40 (#sc-13128, Santa Cruz Biotechnology), occludin (#sc-133256, Santa Cruz Biotechnology), or a rat monoclonal antibody against MECA79 (#sc-19602, Santa Cruz Biotechnology) diluted in PBS-T containing 2.5% bovine serum albumin.
Techniques: Staining, Immunohistochemistry